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Caenorhabditis elegans as an indicator of toxicity of Bacillus thuringiensis strains to Meloidogyne incognita race 3 Ciência Rural
Montalvão,Sandro Coelho Linhares; Castro,Marcelo Tavares de; Soares,Carlos Marcelo Silveira; Blum,Luiz Eduardo Bassay; Monnerat,Rose Gomes.
ABSTRACT: The cotton plant (Gossypium hirsutum) is affected by several diseases of economic importance, among them root-knot nematode (Meloidogyne incognita races 3 and 4). Methods to control this disease include the application of nematicides, solarization, deep plowing, crop rotation and use of antagonistic microorganisms. Among species of Bacillus, there are strains that act as bioregulators and antagonists of several pathogens. Tests to identify these strains are hampered by the difficulty of obtaining large populations of the pathogen and by the time of execution of the in vivo tests that should be conducted for about 90 days. The objective of this research was to compare the toxicity of B. thuringiensis strains to two nematodes, M. incognita and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Phytonematode; Root-knot nematode; Rhizobacteria.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700151
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Screening of Bacillus thuringiensis strains effective against mosquitoes PAB
Monnerat,Rose Gomes; Dias,Daniel Gerhein Souza; Silva,Silvania Ferreira da; Martins,Erica Soares; Berry,Colin; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes; Praça,Lilian Botelho; Soares,Carlos Marcelo Silveira.
The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Culex quinquefasciatus; Aedes aegypti; Bioinsecticide.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000200001
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